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dc.contributor.authorArab, Nagla
dc.contributor.otherQueen's University (Kingston, Ont.). Theses (Queen's University (Kingston, Ont.))en
dc.date.accessioned2018-06-12T18:03:34Z
dc.date.available2018-06-12T18:03:34Z
dc.identifier.urihttp://hdl.handle.net/1974/24276
dc.description.abstractOverexpressing a mouse Bax cDNA in yeast cells induces cell death. When heterologously expressed in yeast cells overexpression of a mouse cDNA encoding Bax, human Thyroid Cancer-1 (TC-1) or annexin V (ANXA5) suppresses the Bax induced cell death. My goal was to characterize the anti-Programmed Cell Death (anti-PCD) potential of these human cDNA sequences. In addition to rescuing Bax-expressing cells, I found that TC-1 and ANXA5 are pro-survival sequences that protect yeast from other death-inducing stresses including copper sulfate. TC-1 also prevented the deleterious effects caused by over-expression of a number of endogenous yeast pro-apoptotic sequences, including the YCA1 encoded metacaspase; the YBH3 encoded Bcl-2 Homology domain 3 protein, the NUC1 encoded DNase, as well as the apoptosis-inducing factor encoded by AIF1. Even though the protective effects were more pronounced in rescued cells over-expressing YBH3 and YCA1, TC-1 could still protect yeast mutant strains lacking YBH3 and YCA1 from the growth inhibitory effects of copper sulfate. Our results demonstrate that TC-1 and ANXA5 are pro-survival sequences that are able to attenuate stress-induced programmed cell death.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesCanadian thesesen
dc.rightsQueen's University's Thesis/Dissertation Non-Exclusive License for Deposit to QSpace and Library and Archives Canadaen
dc.rightsProQuest PhD and Master's Theses International Dissemination Agreementen
dc.rightsIntellectual Property Guidelines at Queen's Universityen
dc.rightsCopying and Preserving Your Thesisen
dc.rightsThis publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.en
dc.subjectProgrammed Cell Death (PCD)en_US
dc.subjectApoptosisen_US
dc.subjectPro-Survivalen_US
dc.subjectAnti-Apoptoticen_US
dc.subjectCell Survivalen_US
dc.subjectYeasten_US
dc.subjectThyroid Cancer-1 (TC-1)en_US
dc.subjectAnnexin V (ANXA5)en_US
dc.titleAnalysis of Human Thyroid Cancer-1 and Annexin V as Pro-Survival cDNA Sequences in Saccharomyces Cerevisiaeen_US
dc.typeThesisen
dc.description.degreeMaster of Scienceen_US
dc.contributor.supervisorYoung, Paul
dc.contributor.supervisorGreenwood, Michael
dc.contributor.departmentBiologyen_US


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