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Please use this identifier to cite or link to this item: http://hdl.handle.net/1974/1176

Title: Activation of EPAC Inhibits the Aquisition of Nucleus Accumbens Amphetamine Place Preference in a Dose-Dependent Manner in Rats
Authors: Park, Sung Woo (Calvin)

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Keywords: dopamine
reward
reward-related learning
addiction
conditioned place preference
place conditioning
cAMP
PKA
Epac
intracellular signalling
Sp-cAMPS
8-pCPT
amphetamine
nucleus accumbens
Issue Date: 2008
Series/Report no.: Canadian theses
Abstract: Reward-related learning occurs when previously neutral stimuli acquires an enhanced ability to elicit approach and other responses. Studies in the past have shown that dopamine receptor-mediated 3’,5’-cyclic adenosine monophosphate (cAMP)-dependent intracellular signalling is important for reward-related learning. Until recently, cAMP-dependent protein kinase (PKA) was the only known signalling molecule that was activated by cAMP. However, it has been discovered that another enzyme, exchange protein directly activated by cAMP (Epac), is also activated by cAMP. Thus, it is possible that cAMP mediates reward-related learning by an Epac-dependent signalling pathway. The present study used a conditioned place preference (CPP) paradigm to investigate whether Epac is involved in the acquisition of reward-related learning. Bilateral injections of amphetamine (20 µg/0.5μl/side) into the nucleus accumbens (NAc) have been shown in previous studies to reliably produce a CPP. Thus, amphetamine (20 µg) and Sp-adenosine 3’,5’-cyclic monophosphorothioate triethylamanine (Sp-cAMPS) (0.1, 1.0, 10, 15, 20 µg), an agent that activates both PKA and Epac, or amphetamine (20 µg) and 8-(4-chlorophenylthio)-2’-O-methyladenosine-3’,5’-cyclic monophosphate (8-pCPT) (0.73, 1.27, 1.45, 2.89, 5.78, 11.56 µg), an agent that selectively activates Epac, were co-injected into NAc to determine their effects on the acquisition of CPP. Results showed that 8-pCPT (1.45 µg), but not lower or higher doses, inhibited CPP. Sp-cAMPS (0.1, 15, 20 µg) also inhibited CPP, replicating the results of previous studies. The results implicate Epac in the acquisition of reward-related learning.
Description: Thesis (Master, Neuroscience Studies) -- Queen's University, 2008-04-25 13:29:37.857
URI: http://hdl.handle.net/1974/1176
Appears in Collections:Neuroscience Studies Graduate Theses
Queen's Theses & Dissertations

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