Queen's University - Utility Bar

QSpace at Queen's University >
Theses, Dissertations & Graduate Projects >
Queen's Theses & Dissertations >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1974/6868

Title: Towards Highly-Reactive Pyri(mi)dinol-Based Fluorescent Antioxidant Indicators And Cyclopropane Lipids: Autoxidizability and Potential as Inhibitors of Lipoxygenases
Authors: YANG, JIANXING

Files in This Item:

File Description SizeFormat
Yang Jianxing 201111 MSc.pdf1.53 MBAdobe PDFView/Open
Keywords: pyridinol pyrimidinol antioxidants
lipoxygenase inhibitors
cyclopropane fatty acids
BODIPY
Issue Date: 11-Nov-2011
Series/Report no.: Canadian theses
Abstract: Chapter 2 In solution, py(mi)ridinols 1.33, 1.34 and 1.35 are 2-, 5- and 28-fold more reactive antioxidants, respectively, than α-TOH (the most potent lipid-soluble antioxidant in nature). In order to develop a highly-reactive fluorescent indicator of lipid peroxidation in cells, we sought to couple these antioxidants with boron-dipyrro- methene (BODIPY) dyes, such that the resulting conjugates will display a significant fluorecence enhancement upon oxidation. This chapter details efforts towards the synthesis of these compounds. Chapter 3 Lipoxygenases are a family of important enzymes that catalyze the dioxygenation of arachidonic acid to yield a variety of potent lipid mediators that have been implicated in the pathogenesis of numerous degenerative conditions. We have undertaken a preliminary study of the effect of replacing the unsaturation in the related polyunsaturated lipid linoleic acid with cyclopropane rings on both the oxidizability of the lipid, as well as lipoxygenase’s ability to utilize it as a substrate. We anticipate that these analogs will be useful in co-crystallization studies with the enzyme that will provide unique insight into substrate acquisition, binding and the necessary conformation for catalysis.
Description: Thesis (Master, Chemistry) -- Queen's University, 2011-11-10 16:15:05.643
URI: http://hdl.handle.net/1974/6868
Appears in Collections:Queen's Theses & Dissertations
Chemistry Graduate Theses

Items in QSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

  DSpace Software Copyright © 2002-2008  The DSpace Foundation - TOP