• Login
    View Item 
    •   Home
    • Graduate Theses, Dissertations and Projects
    • Queen's Graduate Theses and Dissertations
    • View Item
    •   Home
    • Graduate Theses, Dissertations and Projects
    • Queen's Graduate Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Transcriptional Response of Bioenergetic Genes to Exercise

    Thumbnail
    View/Open
    Harris_Shawn_R_201512_MSc.pdf (1.033Mb)
    Date
    2015-12-10
    Author
    Harris, Shawn
    Metadata
    Show full item record
    Abstract
    Transcriptional responses to stressors are typically measured as changes in mRNA levels, despite a broad awareness that transcript levels do not equate to transcription rates. I was able to use goldfish (Carassius auratus) to assess the impact of a single exercise bout on transcription and transcript levels by measuring unprocessed mRNA (pre-mRNA) and mature mRNA levels for critical bioenergetics genes in red and white muscle tissue. I examined housekeeping genes (β-actin: BACT and tubulin: TUB) and select genes for mitochondrial metabolism (citrate synthase: CS and cytochrome c oxidase subunit 4-1: COX4-1), and hexokinase II: HXK. In red muscle, I found that the transcript level for mitochondrial genes (CS and COX4-1) and the housekeeping gene BACT does not change significantly regardless of exercise intensity, but that there is an increase in pre-mRNA levels for these genes at the highest exercise intensity. HXK mRNA showed complex patterns. The mature HXK mRNA increased after a 3 h bout of exercise at the highest exercise intensity (in red and white muscle) but only in recovery after exhaustive exercise in white muscle. The increases in HXK pre-mRNA occurred following exercise and after recovery (in red muscle) and after a period of recovery (in white muscle). In red muscle, exercise didn’t increase the mRNA for any gene within a recovery period of 1 h, except exercise at 80% Ucrit. Analysis of white muscle tissue after a 3 h bout of exercise indicated that there was no change in COX4-1 mRNA post exercise or after recovery, a slight decrease in BACT mRNA after recovery, a significant increase in CS mRNA after recovery and a significant increase in HXK mRNA post exercise. Overall, I found that: the levels of pre-mRNA changed more dramatically than did mature mRNA, the greatest changes occurred at the highest exercise intensity, the increases were mainly seen in recovery rather than during exercise, and there was a greater response in the pathways and tissues that are recruited during the exercise activity.
    URI for this record
    http://hdl.handle.net/1974/13869
    Collections
    • Queen's Graduate Theses and Dissertations
    • Department of Biology Graduate Theses
    Request an alternative format
    If you require this document in an alternate, accessible format, please contact the Queen's Adaptive Technology Centre

    DSpace software copyright © 2002-2015  DuraSpace
    Contact Us
    Theme by 
    Atmire NV
     

     

    Browse

    All of QSpaceCommunities & CollectionsPublished DatesAuthorsTitlesSubjectsTypesThis CollectionPublished DatesAuthorsTitlesSubjectsTypes

    My Account

    LoginRegister

    Statistics

    View Usage StatisticsView Google Analytics Statistics

    DSpace software copyright © 2002-2015  DuraSpace
    Contact Us
    Theme by 
    Atmire NV