Browsing Pathology and Molecular Medicine, Department of by Publish Date
Now showing items 1-20 of 121
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Protein Kinase A, Exchange Protein Activated By cAMP 1, and Phosphodiesterase 4D All Associate With VE-Cadherin to Regulate Endothelial Barrier Function
(2007-09-17)Vascular endothelial cells (VECs) play an essential role in regulating the passage of macromolecules and cells between the blood stream and underlying tissues. The second messenger 3’, 5’ cyclic adenosine monophosphate ... -
Characterizing ErbB2-induced mammary tumourigenesis
(2007-09-18)Approximately 30% of human breast cancers demonstrate overexpression of the receptor tyrosine kinase ErbB2/HER2/Neu, with these cases correlating with recurrence and poor prognosis. While therapy targeting ErbB2 has met ... -
Functional analysis of single nucleotide polymorphisms in the proximal promoter regions of the multidrug transporter genes MRP1/ABCC1 and MRP4/ABCC4
(2007-09-28)The ATP-binding cassette (ABC) transporter superfamily consists of 49 members, to which both Multidrug Resistance Protein 1 (MRP1/gene symbol: ABCC1) and MRP4 (ABCC4) belong. Single nucleotide polymorphisms (SNPs) in drug ... -
Functional consequences of the direct physical interaction between E2A transcription factors and CBP/p300
(2007-10-01)The E2A locus is involved in chromosomal translocations associated with acute lymphoblastic leukemia. The most common of these involves a translocation between chromosomes 1 and 19 (t1;19), resulting in expression of the ... -
Cloning and Functional Characterization of the Retinoic Acid-Catabolizing Enzyme CYP26B1 in Mouse Development
(2007-10-01)Retinoic acid (RA) is an active metabolite of vitamin A that is essential for embryonic development, and homeostasis of adult tissues. RA is a ligand for the nuclear retinoic acid receptor, and RA-mediated signaling is ... -
Molecular Stress Signaling in Breast Epithelial Cells
(2008-01-15)Breast cancer is a complex disease, whose etiology is not well understood. A number of factors have been found to contribute to its development. Psychological stress has been recognized as such a factor in epidemiological ... -
Development of a novel screen protocol for the identification of genes causing replication associated genomic instability in Schizosaccharomyces pombe
(2008-06-04)Replication fork stalling is a source of potentially tumourigenic genomic instability. The RecQ family helicase, Rqh1, is critical for the prevention of replication fork collapse and the formation of potentially deleterious ... -
Identifying a Prognostic Test in Follicular Lymphoma Using a Tissue Microarray and Immunohistochemistry
(2008-07-08)Follicular lymphoma (FL) is an attractive model for discovering biomarkers and elucidating mechanisms of tumour progression. We hypothesized that alterations in the expression of proteins with known roles in cancer biology ... -
Characterizing the function of the Fps/Fes tyrosine kinase in the mammary gland
(2008-07-08)The fps proto-oncogene encodes a 92 kDa cytoplasmic tyrosine kinase. Previous studies have shown that Fps expression in the mammary gland changes with development, and Fps has a suppressor function in mammary tumorigenesis. ... -
Investigating the relationship between the RET receptor, Cbl and ARHGEF7 in downregulation of RET
(2008-07-15)The RET proto-oncogene encodes a receptor tyrosine kinase (RTK), with two major isoforms, RET9 and RET51, which differ in their C-termini, and therefore recruit different signaling complexes. RET plays an important role ... -
FVIII Immunity : early events and tolerance mechanisms to FVIII
(2008-08-15)Among the complications of current treatments for hemophilia A, the development of anti-FVIII antibodies including “FVIII inhibitors” remains the major clinical problem in treating hemophiliacs. Factor VIII inhibitors work ... -
The Mammary Epithelial Cell-Specific Role of Peroxisome Proliferator-Activated Receptor (PPAR)γ in DMBA-Mediated Breast Tumourigenesis
(2008-09-04)Breast cancer is the most commonly diagnosed cancer, and the second leading cause of cancer-related deaths, among women world-wide. Improved understanding of breast tumourigenesis may facilitate the development of more ... -
Role of Cell Adhesion Microenvironment and the Src/Stat3 Axis in Autocrine HGF Signaling During Breast Tumourigenesis
(2008-09-22)Over-expression of both hepatocyte growth factor (HGF) and its receptor Met frequently occurs in invasive human breast cancer, suggesting that the establishment of an HGF “autocrine loop” may be linked to breast tumour ... -
Molecular Characterization of Human Homologs of Yeast MOB
(2008-09-24)MOB (Mps One Binder) is a conserved gene family found in all major kingdoms. The MOB genes are essential components acting in mitotic exit and cytokinesis in both budding and fission yeasts. They are further identified as ... -
Examining the effect of CBP on the E2A-PBX1 and HOXB4 interaction
(2008-09-29)The E2A-PBX1 fusion gene results from the t(1;19) chromosomal translocation that is found in 25% of pre-B-cell cases of acute lymphoblastic leukemia (ALL). The resulting encoded product contains the transactivation domains ... -
Application of Cysteine Scanning Mutagenesis to the Multidrug Resistance Protein (MRP)1
(2009-02-17)Multidrug resistance protein (MRP)1, a member of the ABCC branch of the ATP-binding cassette (ABC) superfamily of transporters, can confer resistance to a broad spectrum of chemotherapeutic agents. In addition to the core ... -
Evaluation of RNA Quality From Formalin Fixed and Paraffin Embedded Samples: Applications and Limitations
(2009-04-14)RNA molecules isolated from FFPE samples are highly fragmented and modified, and generally deemed unsuitable for downstream gene expression profiling. With the development of molecular biology, there has been growing ... -
The Identification of BRCA1 and BRCA2 Mutation Carriers Using Functional Genomic Assays
(2009-04-14)An estimated 5-10% of breast cancers are hereditary in nature and are due to the presence of a mutation in a breast cancer predisposition gene; approximately half of these cases possess a mutation in BRCA1 or BRCA2. Many ... -
The role of Stat3 in cell division and apoptosis
(2009-04-27)The Signal Transducer and Activator of Transcription-3 (Stat3) is a transcription factor that is required for transformation by a number of oncogenes, while a constitutively active form of Stat3 alone is sufficient to ... -
Using Small Molecules to Inhibit an E2A-PBX1:CBP Interaction Involved in Acute Lymphoblastic Leukemia
(2009-09-03)E2A-PBX1 is expressed as a consequence of a recurring chromosomal translocation seen in 5% of acute lymphoblastic leukemia cases. We recently reported that substitution of a leucine residue (L20A) within the N-terminal ...