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dc.contributor.authorAlsaadi, Ftoonen
dc.description.abstractCanada has experienced a significant increase in the transport of diluted bitumen (dilbit), a predominant oil sands product that is created by combining bitumen with diluents derived from oil-gas condensates and other proprietary compounds. The chemical composition of dilbit varies to meet season and transport conditions. While the toxicological effects of a variety of crude and refined oils are well studied, the toxicity of dilbit to fish embryos, which are immobile and thus at a high a risk of exposure to oil in the event of a spill remain largely unknown. To fill this gap, this study assessed the toxicity of two Winter dilbit blends, Access Western Blend (AWB) and Cold Lake Blend (CLB), to fathead minnow (Pimephales promelas) embryos. Embryos were exposed to a range of water accommodated fractions (WAF) and chemically enhanced WAF (CEWAF; using Corexit®9500A as dispersant) for both AWB and CLB. After exposure, hatched fish were assessed for blue sac disease (BSD) and expression of selected biomarker genes. Developmental malformations were observed more frequently with increasing concentrations of AWB and CLB in both WAF and CEWAF treatments and included tube heart, pericardial edema, and craniofacial deformities. Levels of cyp1a and gst mRNA were elevated with increasing total concentrations of polycyclic aromatic hydrocarbons in both AWB and CLB WAF and CEWAF conditions. However, there were no significant changes in mRNA levels for p53, sod, cat, and gsr. These results suggest that expression of cyp1a and gst may serve as biomarkers for dilbit exposure in fathead minnow, furthering our understanding of dilbit-responsive indicators of toxicity in fish species native to North America. This study is important as it utilizes the same exposure methodology to examine the toxicity of two commonly used Canadian dilbits, facilitating the comparison of dilbit toxicity.en
dc.relation.ispartofseriesCanadian thesesen
dc.rightsCC0 1.0 Universalen
dc.rightsQueen's University's Thesis/Dissertation Non-Exclusive License for Deposit to QSpace and Library and Archives Canadaen
dc.rightsProQuest PhD and Master's Theses International Dissemination Agreementen
dc.rightsIntellectual Property Guidelines at Queen's Universityen
dc.rightsCopying and Preserving Your Thesisen
dc.rightsThis publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.en
dc.subjectAccess Western Blenden
dc.subjectCold Lake Blenden
dc.subjectPolycyclic Aromatic Hydrocarbons (PAHs)en
dc.subjectBlue Sac Diseaseen
dc.subjectOxidative Stressen
dc.titleThe Effects of Diluted Bitumen on Developing Fathead Minnow (Pimephales Promelas)en
dc.contributor.departmentBiologyen's University at Kingstonen

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CC0 1.0 Universal
Except where otherwise noted, this item's license is described as CC0 1.0 Universal