Characterization of the interactions of a lignostilbene alpha-beta dioxygenase with both stilbene and carotenoid substrates
As one of many evolutionary compensations for immobility, plants synthesize an array of natural products and metabolites which afford advantage against environmental stresses. The utility of these products is exemplified by stilbene and carotenoid-derived metabolites which are important in plant processes such as immune response and protection against UV-light damage. Not limited to plants, these metabolites even play roles in the maintenance of human health as antioxidants and in vision. Stilbene and carotenoid derivatives are generated by a family of non-heme, iron-coordinating enzymes, divided into two functionally distinct sub-families, including lignostilbene cleavage dioxygenases (LCDs) and carotenoid cleavage oxygenases (CCOs). While these enzymes have highly similar structure and identical reaction mechanisms, LCDs and CCOs have widely different substrate specificities, mediated by factors that currently remain enigmatic. To gain insight into these factors, a previously characterized bacterial LCD from the bio-control agent Pseudomonas brassicacearum (PbLSD) was compared to an array of CCOs by amino acid sequence and structural alignments, as well as in silico docking. This yielded several target residues predicted to contribute to substrate selectivity within the binding pocket. These predictions were tested using an in vivo carotenoid-cleavage assay system combined with site-directed mutagenesis. Current results emphasize that the structure of the binding pocket plays a key role in mediating substrate specificities, with potential applications for the commercial production of desirable carotenoid derivatives. Additional work characterizing specificities and reaction products of purified PbLSD-mutants in vitro, by liquid chromatography and mass spectroscopic methods is ongoing.
URI for this recordhttp://hdl.handle.net/1974/24890
Request an alternative formatIf you require this document in an alternate, accessible format, please contact the Queen's Adaptive Technology Centre
The following license files are associated with this item: