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dc.contributor.authorPyke, Kyra Ellen, 1977-
dc.contributor.otherQueen's University (Kingston, Ont.). Theses (Queen's University (Kingston, Ont.))en
dc.date2007-08-16 18:11:18.941en
dc.date.accessioned2007-08-29T17:10:58Z
dc.date.available2007-08-29T17:10:58Z
dc.date.issued2007-08-29T17:10:58Z
dc.identifier.urihttp://hdl.handle.net/1974/650
dc.descriptionThesis (Ph.D, Kinesiology & Health Studies) -- Queen's University, 2007-08-16 18:11:18.941en
dc.description.abstractAn increase in blood flow associated shear stress results in an endothelial dependent increase in vessel diameter (flow mediated vasodilation (FMD)). Assessment of FMD can provide an index of endothelial function. The stimulus profiles that have been used to investigate FMD in human conduit arteries fall into two categories: reactive hyperemia and sustained stimuli. Stimulus-response specificity proposes that the stimulus characteristics (e.g. magnitude, pattern) are essential determinants of the response characteristics (e.g. magnitude, mechanisms). Purpose: To investigate four specific aspects of FMD stimulus-response specificity: 1) The relative importance of the peak vs. the duration of reactive hyperemia in determining FMD response magnitude. 2) The nitric oxide (NO) dependence of FMD following different durations of reactive hyperemia. 3) The impact of sustained shear stress stimulus magnitude on FMD response dynamics and magnitude. 4) FMD dynamics and magnitude in response to steady vs. oscillatory shear stress evoked passively or via exercise. Methods: Doppler ultrasound was applied to the brachial or radial artery to measure blood flow velocity. Vessel diameter was measured with automated edge detection software. Shear rate, an estimate of shear stress was calculated as the blood flow velocity/vessel diameter. Results: 1) The duration of reactive hyperemia is an important determinant of peak FMD magnitude while the independent contribution of the peak shear to FMD is minimal. 2) NO is not obligatory to FMD following either a five or a ten minute duration occlusion. 3) FMD in response to a sustained stimulus is characterized by a generally bi-phasic response with a fast first phase followed by a slower final phase. 4) The endothelium transduces the mean shear stress when it is exposed passive or handgrip exercise induced oscillations in shear stress. Conclusions: The results indicate that future reactive hyperemia studies must account for the stimulus duration when interpreting FMD results. Further, they demonstrate that the role of NO in FMD is unclear and caution against oversimplified conceptual models of FMD mechanisms. FMD in response to sustained stimuli provides information distinct from reactive hyperemia investigations and exercise may provide a valuable stimulus creation technique.en
dc.format.extent4824952 bytes
dc.format.mimetypeapplication/pdf
dc.languageenen
dc.language.isoenen
dc.relation.ispartofseriesCanadian thesesen
dc.rightsThis publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.en
dc.subjectEndothelial functionen
dc.subjectFlow mediated dilationen
dc.titleStimulus-response specificity of human conduit artery flow mediated dilationen
dc.typeThesisen
dc.description.degreePh.Den
dc.contributor.supervisorTschakovsky, Michael E.en
dc.contributor.departmentKinesiology and Health Studiesen


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