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    Development of a Biomimetic Scaffold for Ligament Tissue Engineering

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    Hayami_James_WS_200611_MASc.pdf (3.830Mb)
    Date
    2011-06-22
    Author
    Hayami, James W. S.
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    Abstract
    The focus of this thesis was to design a scaffold for in vitro culture that would mimic the structure of the native ligament in order to influence primary ligament cells towards the production of ligament-specific tissue. A major part of this project was material selection and subsequent testing to determine if the chosen materials were suitable for the scaffold design. A 20:80 (CL:DLLA) poly(ε-caprolactone-co-D,L-lactide) copolymer (PCLDLLA) was synthesized and electrospun with sub-cellular fibre diameters. The fibres were manufactured into aligned arrays to mimic the collagen fibrils of the ligament. To enhance cell and protein adhesion properties, the PCLDLLA polymer surface was modified using a base catalyzed etching technique. A photocrosslinked

    methacrylated glycol chitosan (M-GC) hydrogel was used to deliver encapsulated

    ligament cells to the biomimetic scaffold and mimic the hydrated proteoglycan matrix

    portion of the ligament. The scaffolds were cultured in vitro for a 4 week period and

    characterized using immunohistochemistry to identify and localize ligament specific

    proteins produced within the scaffolds. Cell culture results indicated that the M-GC

    hydrogel was an effective method of delivering viable cells evenly throughout the

    biomimetic scaffold. Compared to the unmodified PCLDLLA surfaces, the base-etched

    electrospun PCLDLLA fibre surfaces increased cell adhesion and acted as new tissue

    growth guides in the biomimetic scaffold. The biomimetic scaffolds produced and

    accumulated ligament specific proteins: collagens type I and III. The biomimetic scaffold design was determined to be a viable alternative to the current designs of ligament tissue engineering scaffolds.
    URI for this record
    http://hdl.handle.net/1974/6567
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    • Department of Chemical Engineering Graduate Theses
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