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dc.contributor.authorSteele, Joseph Allan McKinnonen
dc.date2011-08-18 15:05:50.917
dc.date.accessioned2011-08-24T15:37:52Z
dc.date.available2011-08-24T15:37:52Z
dc.date.issued2011-08-24
dc.identifier.urihttp://hdl.handle.net/1974/6667
dc.descriptionThesis (Master, Chemical Engineering) -- Queen's University, 2011-08-18 15:05:50.917en
dc.description.abstractA method was developed to produce and incorporate a network of discrete, genipin-crosslinked gelatin microfibers around a pancreatic islet within a barium alginate microcapsule. This technique allows for the encapsulation of a porous fibrous matrix without the geometrical restrictions required for cellular aggregate seeding. Microfibers were produced from a novel vortex-drawn extrusion system with an alginate support matrix. Optimization culminated in a hydrated fiber diameter of 22.3 ± 0.4 μm, a 98% reduction in cross sectional area, while making the process more reliable and less labour intensive. The optimized microfibers were encapsulated at 40 vol% within 294 ± 4 μm 1.6% barium alginate microparticles by an electrostatic-mediated dropwise extrusion system. Pancreatic islets extracted from Sprague Dawley rats were encapsulated within the microparticles, and analyzed over a 21-day preliminary in vitro study. Acridine orange and propidium iodide fluorescent viability staining and light microscopy indicated a significant increase in viability for the fiber-laden particles relative to fiber-free control particles at days 7, 14, and 21. The fiber-laden system also reduced the incidence of disrupted islet cohesion from 31% to 8% at day 21, and showed evidence of islet-fiber adhesion. Preliminary investigations into insulin secretion and metabolic activity showed no significant difference between test and control groups. Further investigation into benefits of islet encapsulation within an extracellular matrix fiber network will be the subject of future studies with this body of work serving as a foundation. The system developed in this investigation could be developed into a modular scaffold system for tissue engineering beyond the field of islet research.en
dc.language.isoengen
dc.relation.ispartofseriesCanadian thesesen
dc.rightsThis publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.en
dc.subjectGelatinen
dc.subjectBioencapsulationen
dc.subjectGenipinen
dc.subjectMicroparticleen
dc.subjectPancreatic Isletsen
dc.subjectMicrofiberen
dc.subjectAlginateen
dc.titleEncapsulation of Protein Microfiber Networks Supporting Pancreatic Isletsen
dc.typethesisen
dc.description.degreeM.A.Sc.en
dc.contributor.supervisorNeufeld, Ronald J.en
dc.contributor.departmentChemical Engineeringen
dc.degree.grantorQueen's University at Kingstonen


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