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dc.contributor.authorFrank, Danielen
dc.date2012-09-27 11:45:16.478
dc.date.accessioned2012-10-13T18:29:04Z
dc.date.issued2012-10-13
dc.identifier.urihttp://hdl.handle.net/1974/7591
dc.descriptionThesis (Master, Biology) -- Queen's University, 2012-09-27 11:45:16.478en
dc.description.abstractActivation-tagging is a functional genomics technique where strong enhancers are inserted randomly into target genomes to over-activate endogenous genes. Phenotypes of interest can be selected for investigation of genetic factors contributing to the mutant phenotype. From initial screens of a population of activation-tagged potato, a mutant with chocolate-coloured tuber skin has been identified. In this thesis, a novel sequence capture method for identifying T-DNA loci in activation tagged potato was used to characterize chocolate’s single T-DNA insertion locus. Transcriptome analysis of tuber periderm tissue was used to identify major processes occurring in the chocolate mutant. Our data suggest activation of a chitin-binding receptor-like kinase located 65 kb from T-DNA insert may cause activation of immune signaling pathways in chocolate. The present work explores a putative model of transcriptional and cellular responses involved in gain-of-function immune receptor activation. Selectively, these findings illustrate the periderm tissue as an important area of defense charged against biotic and abiotic stresses. Periderm development and anatomy are highly important for tuber storage. Further characterization of potato tuber periderm may contribute knowledge to model periderm systems and have implications for molecular breeding strategies to improve tuber storage quality.en
dc.language.isoengen
dc.relation.ispartofseriesCanadian thesesen
dc.rightsThis publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.en
dc.subjectactivation taggingen
dc.subjectperidermen
dc.subjectpotatoen
dc.subjectfunctional genomicsen
dc.subjectSolanum tuberosumen
dc.titleActivation tagging in Solanum tuberosum: Innate immune activation affects potato tuber periderm developmenten
dc.typethesisen
dc.description.restricted-thesisIn this thesis we have identified several candidate genes that may have agronomically important contributions. Further work in confirming the role of these genes is needed before publications can be made.en
dc.description.degreeM.Sc.en
dc.contributor.supervisorRegan, Sharonen
dc.contributor.supervisorTai, Helenen
dc.contributor.departmentBiologyen
dc.embargo.terms1825en
dc.embargo.liftdate2017-10-12
dc.degree.grantorQueen's University at Kingstonen


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