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dc.contributor.authorYu, Fabian
dc.contributor.otherQueen's University (Kingston, Ont.). Theses (Queen's University (Kingston, Ont.))en
dc.date2012-10-29 11:31:54.764en
dc.date.accessioned2012-10-29T20:07:15Z
dc.date.available2012-10-29T20:07:15Z
dc.date.issued2012-10-29
dc.identifier.urihttp://hdl.handle.net/1974/7616
dc.descriptionThesis (Master, Biology) -- Queen's University, 2012-10-29 11:31:54.764en
dc.description.abstractAxon guidance is the developmental process where developing neurons navigate their processes based on attractive and repulsive cues. C. elegans has been an instrumental model in the study of neurobiology with one of the key benefits being a relatively simple nervous system which comprises of only 302 neurons. The Eph Receptors are a canonical class of axon guidance molecules and in C. elegans there is only Eph receptor VAB-1. To understand axon guidance it is useful to study the mechanosensory neurons, and in particular a pair of neurons called the PLM (Posterior Lateral Microtubule). In this thesis I undertook a series of projects involving new techniques, and identified gene products that may interact with VAB-1 in the PLMs. I demonstrate that the use of the PLM Length and PLM/Body length ratio at the L1 stage offers an improved way of detecting axon guidance phenotypes. I show proof of concept that use of a light induced cell ablation technique can help study the developing nervous system. Further, I show that with the use of a tissue specific RNAi technique the role of lethal genes in axon guidance can be analyzed. Finally I conducted a screen that identified new effectors of the VAB-1 signal transduction pathway.en_US
dc.languageenen
dc.language.isoenen_US
dc.relation.ispartofseriesCanadian thesesen
dc.rightsThis publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.en
dc.subjectelegansen_US
dc.subjectRNAien_US
dc.subjectAxon Guidanceen_US
dc.titleCharacterization of the C. elegans PLM Mechanosensory Neurons at the L1 larval stageen_US
dc.typeThesisen_US
dc.description.degreeMasteren
dc.contributor.supervisorChin-Sang, Ian D.en
dc.contributor.departmentBiologyen


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