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Amino Acid-Type Specific Incorporation of Stable 17O Isotopes into Yeast Ubiquitin
Stable isotopic labeling is an important part of structural biology by multidimensional NMR spectroscopy. Novel stable isotopic labeling methods have continuously been developed to enhance protein NMR spectroscopy. Protein ...
Solid-State 17O NMR as a New Probe to Study Acyl-Enzyme Intermediates
This thesis reports on a combined solid-state 17O NMR and quantum mechanical study of three acyl-enzyme intermediates: p-N,N-dimethylaminobenzoyl-chymotrypsin (DAB-CHT), trans¬¬-¬o-methoxycinnamoyl-chymotrypsin (oMC-CHT), ...