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dc.contributor.authorHofmann, Alexanderen
dc.date2013-07-30 13:49:18.059
dc.date2013-08-02 08:42:06.487
dc.date.accessioned2013-08-08T23:57:37Z
dc.date.available2013-08-08T23:57:37Z
dc.date.issued2013-08-08
dc.identifier.urihttp://hdl.handle.net/1974/8153
dc.descriptionThesis (Master, Anatomy & Cell Biology) -- Queen's University, 2013-08-02 08:42:06.487en
dc.description.abstractEarly pregnancy is characterized by complex interactions between blood vessels, leukocytes, and conceptus-derived trophoblasts within the gestational uterus. Uterine Natural Killer (uNK) cells become the most abundant leukocyte during decidualization and produce a wide array of angiogenic factors, yet little is known regarding their early pregnancy functions. To characterize the role(s) of uNK cells, whole mount in situ immunohistochemistry of live early implant sites was performed. A timecourse examination of murine early pregnancy (virgin, and gd4.5-9.5) implantation sites was performed. Comparison of Gd6.5, 8.5 and 9.5 implant sites from BALB/c+/+ controls (BALB/c) and BALB/c-Rag2-/-Il2rg-/- (alymphoid) identified anomalies that result from the absence of lymphocytes. In alymphoid decidua basalis, mesometrial angiogenesis was widespread but pruning of nascent vessels within alymphoid decidua basalis was deficient. As early gestation progressed, vessels of alymphoid decidua basalis showed no evidence for remodeling. Alymphoid implantation sites showed ~24h delay in uterine lumen closure and embryonic development. To determine if uNK cells would normalize the anomalies observed in alymphoid implantation sites, adoptive cell transfer of NK+ B- T- marrow to alymphoid mice was performed. All of the above anomalies were reversed by adoptive transfer of NK+B-T- marrow. My results suggest that uNK cells support vascular growth and development which ensures the decidua can support the growing conceptus early in pregnancy prior to formation and function of the placenta. Human decidual NK cells may fill similar roles and be important targets for strategies designed to correct intra-uterine growth restriction.en
dc.language.isoengen
dc.relation.ispartofseriesCanadian thesesen
dc.rightsThis publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.en
dc.subjectEarly Pregnancyen
dc.subjectWhole Mount in Situ Immunohistochemistryen
dc.subjectIntra-uterine Growth Restrictionen
dc.subjectUterine Natural Killer Cellsen
dc.subjectAngiogenesisen
dc.titleMouse Uterine Natural Killer Cell Functions During Early Pregnancyen
dc.typethesisen
dc.description.degreeM.Sc.en
dc.contributor.supervisorCroy, B. Anneen
dc.contributor.departmentAnatomy and Cell Biologyen
dc.degree.grantorQueen's University at Kingstonen


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