Development of a whole-cell biosensor for β-lactamase inhibitor discovery
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Authors
Jeffs, Mitchell A.
Gray, Rachel A. V.
Sheth, Prameet
Lohans, Christopher T.
Date
2023-10-02
Type
journal article
Language
en
Keyword
Alternative Title
Abstract
The production of β-lactamases by bacterial pathogens endangers antimicrobial therapy, and new inhibitors for β-lactamases are urgently needed. We report the development of a luminescentbased biosensor that quantifies β-lactamase inhibition in a cellular context, based on the activation of transcriptional factor AmpR following the exposure of bacterial cells to β-lactams. This rapid method can account for factors like membrane permeability and can be employed to identify new β-lactamase inhibitors.
Description
This is an Accepted Manuscript of an article published by The Royal Society of Chemistry in Chemical Communications (Cambridge, England) on 2023-10-02, available at https://doi.org/10.1039/D3CC03583B
Citation
Jeffs, Mitchell A., Rachel A. V. Gray, Prameet M. Sheth, and Christopher T. Lohans. 2023. “Development of a Whole-Cell Biosensor for β-Lactamase Inhibitor Discovery.” Chemical Communications (Cambridge, England). doi: 10.1039/D3CC03583B.
Publisher
The Royal Society of Chemistry