Evaluating Changes in Human Skeletal Muscle Protein Content in Response to Unilateral Resistance Exercise Training and Single-Leg Immobilization
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Authors
Boileau, Kristine
Date
2025-03-31
Type
thesis
Language
eng
Keyword
Resistance training , Immobilization , Skeletal muscle , Muscle protein synthesis , Ago2 , Deptor , TP53BP2 , FKBP1A
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Abstract
Background: Skeletal muscle is a large contributor to overall health providing the force required to move as well as serving as a major storage site for ingested glucose and amino acids. Skeletal muscle mass is derived from the balance between muscle protein synthesis (MPS) and muscle protein breakdown (MPB). Both MPS and MPB rates are affected by external stimuli such as resistance exercise and muscle disuse. mTORC1-p70S6K1 and the ubiquitin-proteasome pathways are the most characterized signaling pathways controlling MPS and MPB, respectively. However, novel transcriptomic technology has revealed that resistance exercise and muscle disuse affect the gene expression of the poorly characterized targets Deptor, Ago2, TP53BP2, and FKBP1A. However, little is known about whether resistance exercise and muscle disuse changes the protein content of Deptor, Ago2, TP53BP2, and FKBP1A. Purpose: The purpose of this study is examine if 10 days of unilateral resistance exercise and immobilization alters the protein content of Deptor, Ago2, TP53BP2, and FKBP1A. Hypothesis: We hypothesize that resistance exercise and immobilization will differentially affect the protein expression of Deptor, Ago2, TP53BP2, and FKBP1A in human skeletal muscle. Methods: Seven healthy young females (age: 18-30 years) underwent 10 days of unilateral leg immobilization while the opposing leg completed a total of 4 resistance exercise training sessions. Skeletal muscle biopsies of the vastus lateralis were collected in the rested, fasted state, at baseline and post-intervention in both legs. Western Blotting was used to probe for changes in the content of select proteins informed by retrospective analysis of published transcriptomic data. Results: The protein content of Deptor, Ago2, and TP53BP2, and FKBP1A remained unchanged following both the resistance training and immobilization protocol. Conclusion: Deptor, Ago2, FKBP1A, and TP53BP2 may not be molecular regulators of skeletal muscle in response to resistance training or immobilization.
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ProQuest PhD and Master's Theses International Dissemination Agreement
Intellectual Property Guidelines at Queen's University
Copying and Preserving Your Thesis
This publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.
Attribution-NoDerivatives 4.0 International