The Utility of Electrochemistry - From Surface Modification to Sensor Development
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Authors
McLeod, Jennifer F.
Date
Type
thesis
Language
eng
Keyword
Electrochemistry , Surface Science , Analytical Chemistry , Biosensor , Electroanalysis
Alternative Title
Abstract
Pathogen contamination is an area of concern within water systems, medical settings, food preparation and countless other settings people are immersed in or experience every day. The ability to test for infectious bacteria is critical to maintain a healthy society and prevent the spread of disease. Overwhelmingly, current methods for identifying and detecting bacteria and other pathogens are a time intensive process that necessitates expensive equipment and highly qualified personnel. A fast on-site style test such as the COVID-19 rapid test the world population has become familiar with over the last couple of years, is required to prevent illness particularly in impoverished areas of the world. The following thesis will detail a multifaceted approach towards the development of a cost-effective portable biosensor.
Four different aspects for biosensor design will be presented. Firstly, a complete sensor built on a portable chip with detailed methods for sensor storage will be outlined demonstrating the possibility for on-site pathogen testing with shelf-life capabilities. Secondly, the sensor preparation method will be explored, focusing on the modification of the sensor surface, and outlining a method that has the potential to greatly expedite the time frame for creating a sensor, directly reducing the cost of manufacture. Following, the sensor chip design and construction will be investigated using technologies that allow for in-house budget manufacturing and rapid prototyping. Lastly, an approach to biosensor design will be discussed, highlighting the nuances and challenges associated with sensor development related to the complex nature of the multi-component design in conjunction with complex analytes and matrix.
The development of a broad-spectrum biosensor on a low-cost commercially available screen-printed electrode with specific detection of whole-cell gram-positive bacteria down to 102 CFU / mL detection limit and successful storage strategy for use two weeks post preparation will be described. A time saving methodology for preparing gold surfaces modified with N-heterocyclic carbenes using applied potentials will be detailed. An easily customizable home-built electrode chip that leverages 3-D printing technologies will be defined. Finally, a commentary on the complexities involved in sensor design will be given.
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Queen's University's Thesis/Dissertation Non-Exclusive License for Deposit to QSpace and Library and Archives Canada
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Intellectual Property Guidelines at Queen's University
Copying and Preserving Your Thesis
This publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.
ProQuest PhD and Master's Theses International Dissemination Agreement
Intellectual Property Guidelines at Queen's University
Copying and Preserving Your Thesis
This publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.