Evidence for Mechanical Strains Influence in Osteophyte Development

dc.contributor.authorVenne, Gabrielen
dc.contributor.departmentBiomedical and Molecular Sciencesen
dc.contributor.supervisorPang, Stephen C.en
dc.date.accessioned2016-12-02T14:56:23Z
dc.date.available2016-12-02T14:56:23Z
dc.degree.grantorQueen's University at Kingstonen
dc.description.abstractPurpose: Osteophytes are osteo-cartilaginous metaplastic tissue outgrowths of bone capped by cartilage usually found in degenerative and inflammatory joint disease. The presence and degree of maturity of osteophytes, along with joint space narrowing, are the main radiographic criteria for diagnosis and grading osteoarthritis (OA). Although osteophytes are known for being anatomic signs of advanced OA, they can occur in non-symptomatic joints, in joints with no other observable alterations, and in early stage OA. It remains unclear if they develop from molecular, physiological and/or mechanical stimuli. We hypothesized that mechanical strains play a role in osteophyte development. The overall objective of this thesis was to find evidence that osteophytes are influenced by mechanical strains. Methods: The first project was to develop a mechanically-induced osteophyte animal model. One single impact load that was reported to induce moderate joint damage was applied to the periosteum of the rat knee. Animals were sacrificed at four time points to characterize the evolution of damaged tissue and the joint by histology. A second study using human mature hip osteophytes was conducted to evaluate if mature osteophyte presented histological signs of proliferating and developmental processes. The histological characterization of mature osteophyte was used to compare findings of the mechanically-induced osteophyte in the animal model to validate the use of this rodent model in studying some aspect of osteophyte development of human. Lastly, a detailed three-dimensional (3D) radiological morphometric analysis was performed on microscopic computed tomography (µCT) scanned femoral heads collected from total hip arthroplasty patients presenting mature hip osteophytes. Quantitative morphometric measures of osteophytes internal structure was compared to three regions of the femoral head of known quality of organisation and mechanical constraint. Results and Conclusion: Osteophyte can be mechanically induced by a single load impact to the joint periosteum, indicating that a moderate trauma to the periosteal layer of the joint may play a role in osteophyte development. Mature osteophytes have proliferation, developing and remodelling zones and have trabecular structures. Mechanically-induced osteophytes and mature osteophytes presented similar histological composition. Mature osteophytes have organized internal structure. These results provide evidence that mechanical strain can influence osteophyte development.en
dc.description.degreePhDen
dc.embargo.liftdate2021-11-30T00:50:41Z
dc.embargo.termsWe would request restriction access to the present thesis. We are in the process of publishing materiel included in the presented work. Therefore, we would need to protect rights to commercial publication of materiel. Thanks for understanding. Regardsen
dc.identifier.urihttp://hdl.handle.net/1974/15262
dc.language.isoengen
dc.relation.ispartofseriesCanadian thesesen
dc.rightsQueen's University's Thesis/Dissertation Non-Exclusive License for Deposit to QSpace and Library and Archives Canadaen
dc.rightsProQuest PhD and Master's Theses International Dissemination Agreementen
dc.rightsIntellectual Property Guidelines at Queen's Universityen
dc.rightsCopying and Preserving Your Thesisen
dc.rightsThis publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.en
dc.subjectOsteophyteen
dc.subjectMechanical Strainen
dc.subjectAnimal Modelen
dc.subjectHistologyen
dc.subjectHuman Osteophyteen
dc.subjectMicro-CTen
dc.subjectMorphometryen
dc.titleEvidence for Mechanical Strains Influence in Osteophyte Developmenten
dc.typethesisen
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