Electrical stimulation-induced correlates of epileptogenicity in a non-human primate model of mesial temporal lobe epilepsy
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Authors
Geisler, Riley
Date
2025-01-21
Type
thesis
Language
eng
Keyword
epilepsy , NHP , electrophysiology , primate , afterdischarge , field-evoked potentials , mtle
Alternative Title
Abstract
Mesial temporal lobe epilepsy (mTLE) is a widespread focal seizure disorder, with approximately one-third of patients left untreated by anti-seizure medications (refractory epilepsy) (Kwan and Brodie, 2000). A potential avenue for improving the outcome of surgery and seizure control in refractory epilepsy patients is analyzing changes in electrophysiology throughout the development of epilepsy. In animals, repeatedly stimulating the amygdala (kindling) results in epileptogenic models and produces afterdischarges (ADs) (Goddard et al., 1969). ADs mimic clinical seizure electrophysiology and can potentially be used as a biomarker of tissue epileptogenicity. This study establishes an electrically kindled non-human primate (NHP) model of chronic mTLE with clinical seizure semiology. Epileptogenesis is observed in two NHPs, with significant changes in AD length observed in both primates over months of repeated kindling. Further, reductions in the AD threshold are observed after a seventy-seven-week cessation of kindling, indicating chronicity of kindling-induced changes. The model is further validated by investigating changes in field-evoked potentials recorded throughout kindling using microstimulation, which indicates sensitization of temporal lobe networks to kindling stimulation. Further research in this model has the potential to uncover a method for correlating evoked potential responses to the degree of epileptic activity in primate neural tissue. Future studies should aim to identify similar electrophysiologic findings in human epilepsy patients to refine the neural mapping process of human epilepsy surgery.
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Copying and Preserving Your Thesis
This publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.
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ProQuest PhD and Master's Theses International Dissemination Agreement
Intellectual Property Guidelines at Queen's University
Copying and Preserving Your Thesis
This publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.
Attribution 4.0 International