The Use of Surface Energy Traps as a Platform for Particle Separation and Sample Deposition
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Authors
Jia, Julie
Date
Type
thesis
Language
eng
Keyword
microfluidic , liquid micro junction surface sampling probe , mass spectrometry , particle separation , sample deposition , wettability-patterned microchip , microarray , analyte detection
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Abstract
Microfluidics is the science investigating the behaviour of fluids that are transferred and manipulated through microfluidic channels. It has become a distinct and major technological field that allows for miniaturization of complex systems built into a chip. Digital microfluidics (DMFs), on the other hand, is an alternative approach to channel-based microfluidic systems. Droplets in this case containing reagents or analyte samples are manipulated on two-dimensional planar chips, contrary to microfluidic channels. No or negligible cross-mixing and excellent reagent economy of such systems make them unparalleled. Apart from microfluidics, mass spectrometry (MS) is an important tool in analytical chemistry. Mass spectrometry not only is able to provide compositional information for a sample but has the potential to carry out external calibration by relating the peak area with known concentrations to analyze a sample with unknown concentration. This thesis seeks to use a liquid micro-junction surface sampling probe (LMJ-SSP) as a bridge to connect microfluidics and mass spectrometry together to investigate particle trapping and sample analysis in a more analyte and time efficient way.
A discontinuous de-wetting process is applied on an array of surface energy traps (SETs) which are hydrophilic ablated surfaces along with milled broadening zones (MBZs) formed from the laser ablation of a glass chip coated with hydrophobic coating. By sliding a large droplet on the chip surface, much smaller individual droplets are formed (nanolitre to femtolitre range). The size and shape of the SETs can be simply manipulated by varying the laser micromachining conditions. Ultra-high density is achieved using surface treatments to remove the MBZs such that roughly 5,000 to 45,000 of SETs can be packed on a 25 mm by 75 mm standard microchip glass slide to trap and separate microparticles. To limit sample preparation time and better improve detection efficiency, a microfluidic approach is used with Electrospray Ionization Mass Spectrometry (ESI-MS) coupled with LMJ-SSP for rapid organic/biological sample analysis. The correlation between cell concentration and peak area can be used as a rapid real-time sample detection technique for laboratory purposes.
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Queen's University's Thesis/Dissertation Non-Exclusive License for Deposit to QSpace and Library and Archives Canada
ProQuest PhD and Master's Theses International Dissemination Agreement
Intellectual Property Guidelines at Queen's University
Copying and Preserving Your Thesis
This publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.
Attribution 3.0 United States
ProQuest PhD and Master's Theses International Dissemination Agreement
Intellectual Property Guidelines at Queen's University
Copying and Preserving Your Thesis
This publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.
Attribution 3.0 United States