An automated Genomes-to-Natural Products platform (GNP) for the discovery of modular natural products
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Authors
Johnston, Chad W.
Skinnider, Michael A.
Wyatt, Morgan A.
Li, Xiang
Ranieri, Michael R. M.
Yang, Lian
Zechel, David
Date
2015-09-28
Type
journal article
Language
en
Keyword
Bacteriology , Natural products , Structural biology
Alternative Title
Abstract
Bacterial natural products are a diverse and valuable group of small molecules, and genome sequencing indicates that the vast majority remain undiscovered. The prediction of natural product structures from biosynthetic assembly lines can facilitate their discovery, but highly automated, accurate, and integrated systems are required to mine the broad spectrum of sequenced bacterial genomes. Here we present a genome-guided natural products discovery tool to automatically predict, combinatorialize and identify polyketides and nonribosomal peptides from biosynthetic assembly lines using LC–MS/MS data of crude extracts in a high-throughput manner. We detail the directed identification and isolation of six genetically predicted polyketides and nonribosomal peptides using our Genome-to-Natural Products platform. This highly automated, user-friendly programme provides a means of realizing the potential of genetically encoded natural products.
Description
This article is also available at the publisher's website via https://doi.org/10.1038/ncomms9421. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Citation
Johnston, C., Skinnider, M., Wyatt, M. et al. An automated Genomes-to-Natural Products platform (GNP) for the discovery of modular natural products. Nat Commun 6, 8421 (2015). https://doi.org/10.1038/ncomms9421
Publisher
Springer