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dc.contributor.authorMorrish, Jenna Lee Ellenen
dc.date2008-01-24 10:20:09.589
dc.date.accessioned2008-02-06T14:36:30Z
dc.date.available2008-02-06T14:36:30Z
dc.date.issued2008-02-06T14:36:30Z
dc.identifier.urihttp://hdl.handle.net/1974/1025
dc.descriptionThesis (Master, Chemical Engineering) -- Queen's University, 2008-01-24 10:20:09.589en
dc.description.abstractCarvone is a flavor and fragrance compound that is prominent in nature and is found in the essential oils of many plants. Carvone exists as two enantiomers, (R)-(-)-carvone which has a spearmint aroma and (S)-(+)-carvone which has a caraway aroma and can be used in a variety of applications: as a common food additive, as an antimicrobial/antifungal agent and as a potato sprout inhibitor. Carvone is currently produced by the extraction of essential oils from plants where the yield and quality of the extracted oil depends largely on successful agricultural production of dill, spearmint and caraway plants. Biotechnological production can offer a constant supply of carvone that is independent of several agricultural limitations. In this study, it was confirmed that the substrate and product of the microbial biotransformation of trans-carveol to (R)-(-)-carvone by Rhodococcus erythropolis DCL14 can be inhibitory to the cells at high concentrations. As such, a two phase partitioning bioreactor was employed where the function of the second phase (immiscible organic solvent or solid polymer beads) was to partition the inhibitory substrate into the aqueous phase at a rate governed by the metabolic demand of the cells and uptake the inhibitory product as it accumulated in the aqueous phase. Rational selection strategies were employed when determining the appropriate organic solvent and solid polymer to be used as the second phase. The performance of the reactor was evaluated based on volumetric productivity, length of biotransformation and total volume of substrate added to the reactor. The most successful reactor configuration was one in which styrene/butadiene copolymer beads were used as a second phase in the reactor and the fermentation medium was continuously circulated through an external extraction column packed with Hytrel® 8206 polymer beads. The volumetric productivity, length of biotransformation and total volume of substrate added to this reactor were 99 mg/L.h, 48.75 h and 35 mL, respectively whereas in the single phase benchmark reactor the performance indicators were only 31 mg/L.h, 15.25 h and 5 mL, respectively. These results clearly show the advantage of employing a partitioning bioreactor configuration for the biotechnological production of high value chemical species that exhibit cytotoxicity.en
dc.format.extent1748155 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoengen
dc.relation.ispartofseriesCanadian thesesen
dc.rightsThis publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.en
dc.subjectMicrobial biotransformationen
dc.subjectTwo phase partitioning bioreactoren
dc.titleEnhancement of the microbial biotransformation of (-)-trans-carveol to (R)-(-)-carvone by Rhodococcus erythropolis DCL14 in various two phase partitioning bioreactor configurationsen
dc.typethesisen
dc.description.degreeM.Sc.en
dc.contributor.supervisorDaugulis, Andrew J.en
dc.contributor.departmentChemical Engineeringen
dc.degree.grantorQueen's University at Kingstonen


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