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dc.contributor.authorWeber, Jackie
dc.contributor.otherQueen's University (Kingston, Ont.). Theses (Queen's University (Kingston, Ont.))en
dc.date.accessioned2019-09-25T22:15:35Z
dc.date.available2019-09-25T22:15:35Z
dc.identifier.urihttp://hdl.handle.net/1974/26611
dc.description.abstractThe use of plants to remediate contaminated lands is of growing interest to the scientific community because of its ease of implementation, cost effectiveness and ability to stabilize contaminated soils. A novel Populus tremula x Populus alba mutant named fuzzy with an increased trichome density, an elevated growth rate, enhanced pest resistance and a predicted root phenotype was investigated for its ability to tolerate and accumulate arsenic and cadmium in soil. The chlorophyll, hydrogen peroxide and proline contents of fuzzy tissues were assayed, its tissue metal content was measured using ICP-MS and its wet biomass was quantified. Transgenic Arabidopsis thaliana overexpressing homologous gene of that overexpressed in the fuzzy poplar were grown on gel media and their roots were measured to corroborate the root phenotype of fuzzy poplars. The fuzzy poplar was found to have lower levels of hydrogen peroxide in its tissues after arsenic and cadmium exposure than control poplars, suggesting it has a reduced oxidative stress response when exposed to high levels of arsenic and cadmium. This low hydrogen peroxide content was not due to reduced arsenic and cadmium accumulation in fuzzy trees, as they did not accumulate different amounts of arsenic or cadmium than control plants. Proline and chlorophyll levels were not significantly different between metal treatments, or between fuzzy and control poplars. fuzzy poplars exhibited significantly higher root biomass than control poplars, and transgenic Arabidopsis thaliana overexpressing a homologous gene to the one that causes the fuzzy phenotype had significantly longer roots than control plants. All poplars exposed to arsenic and cadmium in this trial contained the majority of these compounds in their tissues in roots. The root biomass phenotype, low hydrogen peroxide content and root metal accumulation in the fuzzy poplar make it an ideal candidate for bioremediation and soil stabilization of contaminated soils.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesCanadian thesesen
dc.rightsCC0 1.0 Universal*
dc.rightsQueen's University's Thesis/Dissertation Non-Exclusive License for Deposit to QSpace and Library and Archives Canadaen
dc.rightsProQuest PhD and Master's Theses International Dissemination Agreementen
dc.rightsIntellectual Property Guidelines at Queen's Universityen
dc.rightsCopying and Preserving Your Thesisen
dc.rightsThis publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.en
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.subjectBioremediationen_US
dc.subjectPhytoremediationen_US
dc.subjectPoplaren_US
dc.subjectArsenicen_US
dc.subjectCadmiumen_US
dc.titleBioremediation of arsenic and cadmium in a Populus tremula x Populus alba mutanten_US
dc.typethesisen
dc.description.degreeMaster of Scienceen_US
dc.contributor.supervisorRegan, Sharon
dc.contributor.departmentBiologyen_US


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CC0 1.0 Universal
Except where otherwise noted, this item's license is described as CC0 1.0 Universal