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dc.contributor.authorDavies, Rhiannon
dc.contributor.otherQueen's University (Kingston, Ont.). Theses (Queen's University (Kingston, Ont.))en
dc.date2007-09-05 16:14:17.684en
dc.date2007-10-31 11:55:28.757en
dc.date.accessioned2007-11-01T12:28:20Z
dc.date.available2007-11-01T12:28:20Z
dc.date.issued2007-11-01T12:28:20Z
dc.identifier.urihttp://hdl.handle.net/1974/897
dc.descriptionThesis (Master, Biology) -- Queen's University, 2007-10-31 11:55:28.757en
dc.description.abstractThe influence of body size on metabolic rate, muscle enzyme activities, and the underlying patterns of mRNA for these enzymes were explored in an effort to explain the genetic basis of allometric variation in metabolic enzymes. Two pairs of sister species of centrarchid fishes were studied: black bass (largemouth bass, Micropterus salmoides and smallmouth bass, Micropterus dolomieui), and sunfish (pumpkinseed, Lepomis gibbosus and bluegill, Lepomis macrochirus). The goal was to assess the regulatory basis of both intraspecific and interspecific variation in relation to body size, as well as gain insights into the evolutionary constraints within lineages. Whole animal routine metabolic rate showed scaling coefficients not significantly different from 1, ranging from +0.87 to +0.96. However, there were significant effects of body size on the specific activities of oxidative and glycolytic enzymes. Mass-specific activity of the oxidative enzyme citrate synthase (CS) scaled negatively with body size in each species, with scaling coefficients ranging from -0.15 to -0.19 whereas the glycolytic enzyme pyruvate kinase (PK) showed positive scaling, with scaling coefficients ranging from +0.08 to +0.23. The ratio of mass-specific enzyme activity in PK to CS increased with body size, whereas the ratio of mRNA transcripts of PK to CS was unaffected, suggesting the enzyme relationships were not due simply to transcriptional regulation of both genes. The mass-dependent differences in PK activities were best explained by transcriptional regulation of the muscle PK gene; PK mRNA was a good predictor of PK specific enzyme activity within species and between species. Conversely, CS mRNA did not correlate with CS specific enzyme activities, suggesting post-transcriptional mechanisms may explain the observed inter-specific and intraspecific differences in oxidative enzymes.en
dc.format.extent249999 bytes
dc.format.mimetypeapplication/pdf
dc.languageenen
dc.language.isoenen
dc.relation.ispartofseriesCanadian thesesen
dc.rightsThis publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner.en
dc.subjectAllometryen
dc.subjectPyruvate Kinaseen
dc.subjectMetabolismen
dc.subjectWhite Muscle Energeticsen
dc.subjectCitrate Synthaseen
dc.titleAllometric Scaling in Centrarchid Fish: Origins of Intra- and Inter-specific Variation in Oxidative and Glycolytic Enzyme Levels in Muscleen
dc.typethesisen
dc.description.degreeMasteren
dc.contributor.supervisorWang, Yuxiang S.en
dc.contributor.supervisorMoyes, Christopher D.en
dc.contributor.departmentBiologyen


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